Coding
Part:BBa_K2950003:Design
Designed by: Shuxin Xiao Group: iGEM19_BNDS_China (2019-10-16)
toluene dihydrodiol dehydrogenase(TodD) in P.putida
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 381
Illegal NgoMIV site found at 469
Illegal AgeI site found at 736 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to dehydrogenate our intermediate cis-hexadecylbenzene dihydrodiol to urushiol, we utilized todD in toluene dioxygenase pathway to reduce two hydrogens on the adjacent carbons to reform a benzene ring.
Source
Pseudomonas putida