Coding

Part:BBa_K2950003:Design

Designed by: Shuxin Xiao   Group: iGEM19_BNDS_China   (2019-10-16)


toluene dihydrodiol dehydrogenase(TodD) in P.putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 381
    Illegal NgoMIV site found at 469
    Illegal AgeI site found at 736
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to dehydrogenate our intermediate cis-hexadecylbenzene dihydrodiol to urushiol, we utilized todD in toluene dioxygenase pathway to reduce two hydrogens on the adjacent carbons to reform a benzene ring.


Source

Pseudomonas putida

References